Protein_Domain

Part:BBa_K3824000:Design

Designed by: Woo Rin Lee   Group: iGEM21_Korea_HS   (2021-09-28)


Buforin IIB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Peptide sequence:

RAGLQFPVGRLLLRRLLRRLLR (21 aa) net charge (+7)

Buforin II, a cationic antimicrobioal peptides (AMP) with a helix–hinge–helix structure derived from histone H2A, has a unique mechanism of action: It rapidly crosses bacterial membranes without lysing cells; rather, it kills bacteria by interacting with intracellular macromolecules. Buforin IIb is a synthetic analog of buforin II that contains a proline hinge in the middle and a model α-helical sequence at C-terminus (3×RLLR).

The total net charge of amino acid was considered to design this peptide sequence

Source

Synthesized peptide from Anygen a biotech company from South Korea

References

Lee HS, Park CB, Kim JM, Jang SA, Park IY, Kim MS, Cho JH, Kim SC. Mechanism of anticancer activity of buforin IIb, a histone H2A-derived peptide. Cancer Lett. 2008 Nov 18;271(1):47-55. doi: 10.1016/j.canlet.2008.05.041. Epub 2008 Jul 9. PMID: 18617323.

Cho JH, Sung BH, Kim SC. Buforins: histone H2A-derived antimicrobial peptides from toad stomach. Biochim Biophys Acta. 2009 Aug;1788(8):1564-9. doi: 10.1016/j.bbamem.2008.10.025. Epub 2008 Nov 11. PMID: 19041293.